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分析黑 腹果蝇 幼虫脂肪体中饥饿诱导的自噬
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Biologia
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JoVE Journal Biologia
Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body
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分析黑 腹果蝇 幼虫脂肪体中饥饿诱导的自噬

DOI:
10.3791/64282-v

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06:02 min

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August 04, 2022

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1Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Capítulos

  • 00:04Introduction
  • 00:40Drosophila Crossing and Egg Laying
  • 01:54Amino Acid Starvation Induces Autophagy
  • 02:51Third Instar Larvae Dissection and Sample Tissue Processing
  • 04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
  • 05:22Conclusion

Summary

Tadução automática

Tadução automática

本协议描述了通过营养消耗在黑腹果蝇幼虫脂肪体中诱导自噬，并使用转基因苍蝇菌株分析自噬的变化。

Tags

Keywords: AutophagyDrosophila MelanogasterLarval Fat BodyClonal AnalysisAmino Acid DepletionThird Instar Larvae20% Sucrose SolutionDissection

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