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Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum
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Capítulos
- 00:04Introduction
- 01:36EdU Incorportaion and Fixation
- 02:25Fluorescent In Situ Hybridization
- 06:27Results: EdU and Nanos1 Expression Patterns on the Proximal, Adaxial Side of Cladonema Medusa Tentacle
- 08:02Conclusion
Here, we describe a protocol for visualizing stem-like proliferating cells in the jellyfish Cladonema. Whole-mount fluorescent in situ hybridization with a stem cell marker allows for the detection of stem-like cells, and 5-ethynyl-2'-deoxyuridine labeling enables the identification of proliferating cells. Together, actively proliferating stem-like cells can be detected.
Tags
Keywords:
Fluorescent In Situ Hybridization5-ethynyl-2′-deoxyuridine LabelingStem-like CellsHydrozoan JellyfishCladonema PacificumNanos1 ExpressionEdU LabelingStem Cell HeterogeneityBiologia do DesenvolvimentoRegenerationAdult Stem CellsTentacle BranchingTentacle RegenerationLaboratory Model Organism
