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太平洋水母干细胞样细胞的荧光原位杂交和5-乙炔基-2'-脱氧尿苷标记

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum

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太平洋水母干细胞样细胞的荧光原位杂交和5-乙炔基-2'-脱氧尿苷标记

DOI:

10.3791/64285-v

•

08:44 min

•

August 03, 2022

•

Sosuke Fujita, Erina Kuranaga, Masayuki Miura, Yu-ichiro Nakajima

¹Graduate School of Life Sciences,Tohoku University, ²Graduate School of Pharmaceutical Sciences,The University of Tokyo

Capítulos

00:04Introduction
01:36EdU Incorportaion and Fixation
02:25Fluorescent In Situ Hybridization
06:27Results: EdU and Nanos1 Expression Patterns on the Proximal, Adaxial Side of Cladonema Medusa Tentacle
08:02Conclusion

Summary

Tadução automática

在这里，我们描述了一种用于可视化水母 Cladonema中干细胞样增殖细胞的协议。与干细胞标记物的全安装荧光原位杂交可以检测干细胞样细胞，5-乙炔基-2'-脱氧尿苷标记可以鉴定增殖细胞。一起，可以检测到活跃增殖的干细胞样细胞。

Tags

Keywords: Fluorescent In Situ Hybridization 5-ethynyl-2′-deoxyuridine Labeling Stem-like Cells Hydrozoan Jellyfish Cladonema Pacificum Nanos1 Expression EdU Labeling Stem Cell Heterogeneity Biologia do Desenvolvimento Regeneration Adult Stem Cells Tentacle Branching Tentacle Regeneration Laboratory Model Organism

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