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Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
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Neurociência
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JoVE Journal Neurociência
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

DOI:
10.3791/64286-v

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07:13 min

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February 10, 2023

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, ,
1Department of Audiology and Speech-Language Pathology,MacKay Medical College, 2Institute of Biomedical Sciences,MacKay Medical College

Capítulos

  • 00:04Introduction
  • 01:04Induction of Noise‐Induced Hearing Loss (NIHL) in Mice
  • 03:07Auditory Brainstem Response (ABR)‐Based Assessment of Hearing Threshold
  • 04:19Microscope Examination
  • 05:18Results: Assessing Hearing Function and Outer Hair Cell Damage in NIHL Mice
  • 06:32Conclusion

Summary

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Here, we present a protocol for a mouse model of noise-induced hearing loss (NIHL). To induce NIHL, we developed a new and simple device using corrugated plastic, a rat trap cage, and a speaker. Auditory brainstem response and immunofluorescence imaging were employed to assess the hearing function and outer hair cell damage, respectively.

Tags

Keywords: Noise-induced Hearing LossMiceABROuter Hair Cell DamageExperimental ConditionsHearing Function AssessmentAnimal ModelSound ExposureHearing Threshold

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