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Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
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JoVE Journal Química
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

DOI:
10.3791/64289-v

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07:11 min

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September 28, 2022

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, , , , , , , ,
1Pingshan Translational Medicine Center,Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology,Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital,Anhui Medical University

Capítulos

  • 00:04Introduction
  • 00:38N-Terminal Fmoc Deprotection
  • 01:36Coupling the Linear Peptide
  • 02:49Bisalkylation Between Methionine and Cysteine
  • 04:34Propargyl Sulfonium Salt Cyclization
  • 05:21Results: Characterization of the Synthesized Peptides
  • 06:32Conclusion

Summary

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This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

Tags

Keywords: Cyclic PeptideSulfonium CenterCysteineN-terminal DeprotectionPeptide CouplingNinhydrin TestResin WashingTrityl-L-cysteine Deprotection

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