Journal
/
Imunologia e Infecção
/
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

DOI:
10.3791/64291-v

•

08:15 min

•

December 09, 2022

•

, , , , , , , , , , , ,
1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

Capítulos

  • 00:05Introduction
  • 00:55L929 Cytotoxicity Assay
  • 01:51Splenocyte Stimulation
  • 04:02Uptake by DCs
  • 06:09Macrophage Activation
  • 06:57Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
  • 07:52Conclusion

Summary

Tadução automática

Tadução automática

The protocol presents detailed methods for evaluating whether the nanoemulsion ophiopogonin D adjuvant promotes effective cellular immune responses.

Tags

Keywords: In VitroCellular ActivityNanoemulsionVaccine AdjuvantOphiopogonin DCellular ResponseAdjuvant EvaluationL929 CellsDMEMCentrifugationMouse SpleenRed Blood Cell LysisCellular Delivery

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/64291?article_type=v

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
check_url/pt/64291?article_type=v

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
check_url/pt/64291?article_type=v

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
check_url/pt/64291?article_type=v

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
check_url/pt/64291?article_type=v

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
check_url/pt/64291?article_type=v

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
check_url/pt/64291?article_type=v

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
check_url/pt/64291?article_type=v

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
check_url/pt/64291?article_type=v

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo
check_url/pt/64291?article_type=v

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Read Article