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结合透明和荧光显微镜，可视化基因表达的变化和对芸苔疟原虫的生理反应

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

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结合透明和荧光显微镜，可视化基因表达的变化和对芸苔疟原虫的生理反应

DOI:

10.3791/64297-v

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06:58 min

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August 05, 2022

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Deeksha Singh, Sara Blicharz, Karolina Stefanowicz, Laura Ragni, Kornel Michalak, Agnieszka Bagniewska-Zadworna, Robert Malinowski

¹Integrative Plant Biology Team,Institute of Plant Genetics Polish Academy of Sciences, ²ZMBP-Center for Plant Molecular Biology,University of Tübingen, ³Department of General Botany, Institute of Experimental Biology, Faculty of Biology,Adam Mickiewicz University

Capítulos

00:05Introduction
01:36Tissue Preparation and Fixation
02:13Tissue Embedding and Sectioning
03:03Vibratome Sectioning and Specimen Clearing
04:03Staining Procedure and Microscopy
04:49Results: Microscopic Analysis of the Biotrophic Interaction and Gene Expression Regulations Between Plasmodiophora brassicae and Arabidopsis Plants
06:06Conclusion

Summary

Tadução automática

本协议描述了一种优化的方法，用于组织学观察由 芸苔疟原虫 诱导的瘿。在荧光成像之前清除下胚轴的振动切片，以研究疾病进展过程中转录因子和植物激素的参与。该方案克服了树脂包埋的限制 ，使荧光 蛋白的植物可视化成为可能。

Tags

Plasmodiophora Brassicae Clubroot Gene Expression Fluorescence Microscopy Clearing Galls Phytohormone Lignification Plant-pathogen Interaction Agarose Embedding Vibratome Sectioning

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