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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome
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Biologia
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JoVE Journal Biologia
Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

DOI:
10.3791/64298-v

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12:55 min

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January 13, 2023

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,
1Department of Embryology,Carnegie Institution for Science, 2Department of Biology,Johns Hopkins University

Capítulos

  • 00:04Introduction
  • 01:28Homogenizing Flies Individually in a 96-Well PCR Plate
  • 04:13Serial Dilution of Fly Homogenate and Spotting onto CFU Plates
  • 06:03Quantification of CFUs
  • 06:44Automated Colony Counting Using the Count-On-It Suite
  • 09:57Results: Enumeration of CFUs Using Automated Colony Counting
  • 12:10Conclusion

Summary

Tadução automática

Tadução automática

This method quantifies microbial abundance using a 96-well plate format to plate colony forming units (CFUs) and is applied to the Drosophila microbiome in whole fly homogenate samples. CFUs are counted with an automated image analysis software provided here.

Tags

Colony Forming UnitCFUMicrobiomeDrosophilaHigh-throughputAutomated CountingGnotobiotic96-well PlateMicrofluidicsMicrobiologyMicrobial Quantification

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