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Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

DOI:

10.3791/64299-v

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07:33 min

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September 28, 2022

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Zelong Zhang, Dingyi Cai, Shuang Ge, Xing Luo, Xiaoqiang Zeng, Yan Ye, Zhen Song, Liusheng Peng, Haibo Li, Quanming Zou, Hao Zeng, Hongwu Sun, Yun Yang

¹National Engineering Research Centre of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy,Third Military Medical University of Chinese PLA

Capítulos

00:04Introduction
00:49Preparation of the I‐OVA NE
01:33In Vitro and In Vivo Toxicity Assays
05:25Results: In Vitro and In Vivo Experimental Evaluation of the Nasal Tumor Nanovaccine Delivery
07:06Conclusion

Summary

Tadução automática

Here, we present detailed methods for the preparation and evaluation of the nasal self-assembled nanoemulsion tumor vaccine in vitro and in vivo.

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Keywords: Nasal Self-assembled Nanoemulsion Tumor Vaccine In Vitro In Vivo Monophosphorol Lipid A DMSO TWEEN 80 I-OVA Squalene MPLA BEAS2B Epithelial Cells PBS Trypsin RPMI 1640

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