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Immunology and Infection

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JoVE Journal Immunology and Infection
Murine fækal isolering og mikrobiota transplantation
 
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Murine fækal isolering og mikrobiota transplantation

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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Målet her er at skitsere en protokol til at undersøge mekanismerne for dysbiose i hjerte-kar-sygdomme. Dette papir diskuterer, hvordan man aseptisk indsamler og transplanterer murine fækale prøver, isolerer tarmene og bruger "Swiss-roll" -metoden efterfulgt af immunfarvningsteknikker til at forhøre ændringer i mave-tarmkanalen.

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Immunologi og infektion udgave 195 fækal mikrobiotatransplantation schweizisk rulle kardiovaskulær
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