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1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics,Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics,Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology,Vanderbilt University Medical Center
Capítulos
- 00:05Introduction
- 00:29Aseptic Collection of Mouse Fecal Samples
- 01:21Fecal Matter Transplantation (FMT)
- 02:06Systolic Blood Pressure Measurements
- 02:47Assessment of FMT to Cardiovascular Changes and Making Gut Intestine Swiss-Rolls
- 05:46Results: Effects of High and Low-Salt Diet, Fecal Microbiota Transplantation from High-Salt Fed Mice, and Immunohistochemistry on the Ileum
- 07:07Conclusion
Het doel hier is om een protocol te schetsen om de mechanismen van dysbiose bij hart- en vaatziekten te onderzoeken. Dit artikel bespreekt hoe aseptisch muizenfecale monsters kunnen worden verzameld en getransplanteerd, darmen kunnen worden geïsoleerd en de "Swiss-roll" -methode kan worden gebruikt, gevolgd door immunostainingtechnieken om veranderingen in het maagdarmkanaal te onderzoeken.
