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3D多細胞肺腫瘍スフェロイドから分離された癌関連線維芽細胞におけるミトコンドリアの健康評価
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JoVE Journal Pesquisa do Câncer
Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
DOI:

10:26 min

October 21, 2022

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Capítulos

  • 00:04Introduction
  • 01:13Preparation of Cell Suspension
  • 03:24Live-Dead Analysis of Tumor Spheroids
  • 04:05Disintegration and Cell Suspension of Tumor Spheroids
  • 05:02Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
  • 06:19Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
  • 07:28Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
  • 09:30Conclusion

Summary

Tadução automática

多細胞3D腫瘍スフェロイドを肺腺癌細胞、線維芽細胞、および単球を用いて調製し、続いてこれらのスフェロイドから癌関連線維芽細胞(CAF)を単離した。単離されたCAFを正常な線維芽細胞と比較し、ミトコンドリアの膜貫通電位、活性酸素種、および酵素活性を研究することにより、ミトコンドリアの健康状態を評価しました。

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