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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Capítulos
- 00:04Introduction
- 00:44Preparation of Ribozyme
- 01:44GQ-EXPAR
- 02:18Color Development
- 02:45Results: GQ-EXPAR Visual Results
- 03:12Conclusion
The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine.
