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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
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Bioquímica
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JoVE Journal Bioquímica
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

DOI:
10.3791/64360-v

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10:16 min

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January 20, 2023

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, , , , , , ,
1Central Laboratory of Women’s Hospital,Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University, 3Core Facilities,Zhejiang University School of Medicine

Capítulos

  • 00:04Introduction
  • 00:34Cell Sorting
  • 05:40Isolation of Small Extracellular Vesicles (sEV)
  • 08:00Results: Purification and Analysis of Small Extracellular Vesicles (sEV)
  • 09:43Conclusion

Summary

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Tadução automática

This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

Tags

Flow CytometryExtracellular VesiclesSmall Extracellular VesiclesSize-specific SortingHigh-throughput IsolationPurificationFlow Cytometric Sorting ParametersForward ScatterSize ScatterStartup ProcedureLaser PowerSheath FluidSample FlowStream AlignmentLaser InterceptNozzle AlignmentFine Laser AlignmentRainbow Fluorescent ParticlesQuality ControlIntelliSort

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