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一种内部构建的基于发光二极管的光动力治疗设备，用于增强维替泊芬在 2D 细胞培养模型中的细胞毒性

JoVE Journal
Bioquímica
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JoVE Journal Bioquímica

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

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一种内部构建的基于发光二极管的光动力治疗设备，用于增强维替泊芬在 2D 细胞培养模型中的细胞毒性

DOI:

10.3791/64391-v

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11:04 min

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January 13, 2023

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Isadora da Silva Zanzarini, Gustavo Barbosa, Larissa de Oliveira Prado, Ingrid Fatima Zattoni, Gustavo Da Paz, Ademir Luiz do Prado³, Waldemar Volanski, Marcos Dinís Lavarda, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Vivian Rotuno Moure, Glaucio Valdameri

¹Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, ²Graduate Program in Pharmaceutical Sciences,Federal University of Parana, ³Federal Institute of Parana

Capítulos

00:02Introduction
00:40Device Construction
03:10Cell Lines: Cultivation, Seeding, and Treatment
05:19Device Operation
06:56Cell Viability Assays
07:47Representative Results
09:50Conclusion

Summary

Tadução automática

在这里，我们描述了一种新颖，简单且低成本的设备，可以使用二维HeLa细胞培养物和维特泊芬作为光敏剂成功进行体外光动力疗法（PDT）测定。

Tags

Keywords: Photodynamic Therapy PDT Light-emitting Diode LED Verteporfin Cytotoxicity 2D Cell Culture In-house-built Device PhotoAct Low-cost HeLa Cells Dulbecco’s Modified Eagle Medium Cell Culture

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