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使用全基因组测序表征源自Oreochromis spp.农场的致病性大肠杆菌菌株

JoVE Journal
Genética
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JoVE Journal Genética

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

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使用全基因组测序表征源自Oreochromis spp.农场的致病性大肠杆菌菌株

DOI:

10.3791/64404-v

•

09:44 min

•

December 23, 2022

•

José Antonio Magaña-Lizárraga, Bruno Gómez-Gil, Julissa Enciso-Ibarra, María Elena Báez-Flores

¹Unidad de Investigaciones en Salud Pública “Dra. Kaethe Willms”, Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Sinaloa, ²Centro de Investigación en Alimentación y Desarrollo, A. C. (CIAD),Unidad Mazatlán en Acuicultura y Manejo Ambiental

Capítulos

00:04Introduction
01:32DNA Library Preparation
04:15Library Pooling, Denaturalizing, Sequencer Initiating
07:05Sequence Data Analysis
08:07Results: Circular Map and Subsystem Distribution of Draft Genome of E. coli ACM5
09:15Conclusion

Summary

Tadução automática

使用台式仪器进行全基因组测序（WGS）策略的可行性简化了实验室环境中对每种与公共卫生相关的微生物的基因组询问。描述了细菌WGS工作流程的方法学调整，并介绍了用于分析的生物信息学管道。

Tags

Whole-genome Sequencing WGS Antimicrobial Resistance Pathogenic Escherichia Coli Oreochromis Spp. Farms Next-generation Sequencing DNA Extraction Tagmentation Indexing PCR Magnetic Bead Purification

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