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Microdissection of the Rodent Eye
JoVE Journal
Medicina
Author Produced
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JoVE Journal Medicina
Microdissection of the Rodent Eye
DOI:

11:03 min

April 21, 2023

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Capítulos

  • 00:07Introduction
  • 02:16Preparation
  • 03:15Enucleation of the Mouse Eye Along with the Third Eyelid
  • 04:03Clearing the Extraneous Tissue
  • 05:17Dissection of the Enucleated Eye to Obtain Anterior and Posterior Eyecups
  • 07:28Isolation of Neural Retina and RPE Layers
  • 08:16Segmentation of Retinal and RPE Layers for Whole Mounts
  • 09:38Resultados
  • 10:12Conclusion

Summary

Tadução automática

This paper presents a protocol for ocular micro-dissection in rodents. The process involves the enucleation of the eyeball along with the nictitating membrane (i.e., the third eyelid). This is then followed by the separation of the posterior and anterior eye cups.

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