/

/

一种基于人源酵母模型研究α-突触核蛋白毒性和聚集的方法

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Please note that all translations are automatically generated. Click here for the English version.

一种基于人源酵母模型研究α-突触核蛋白毒性和聚集的方法

DOI:

10.3791/64418-v

•

08:24 min

•

November 25, 2022

•

Hyunhee Kim, Juwon Jeong, Changhan Lee

¹Department of Biological Sciences,Ajou University

Capítulos

00:05Introduction
00:48Yeast Transformation and Spotting Assay
02:57Measurement of Yeast Growth Using a Microplate Reader
04:18Fluorescence Microscopy
06:31Results: Cytotoxicity of α-Synuclein on Agar and Liquid Media and Analysis of Yeast Cells by Fluorescence Microscopy
07:28Conclusion

Summary

Tadução automática

需要α-突触核蛋白的体内生理模型来研究和了解帕金森病的发病机制。我们描述了一种使用人源化酵母模型监测α-突触核蛋白的细胞毒性和聚集体形成的方法。

Tags

α-synuclein Yeast Model Parkinson’s Disease Protein Aggregation Cellular Phenotypes Genetic Factors Growth Assay Toxicity

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

人类DNA修复蛋白的遗传研究利用酵母作为模型系统

根据流量的血小板粘附和聚集，使用微流控流细胞

嗨 - C：一个方法研究基因组的三维架构。

生长实验，以评估在酵母中的多聚谷氨酰胺毒性

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

生成一个“人性化”<em>果蝇</em> S2细胞敏感的驱动蛋白-5的药理抑制

方法研究线虫线虫中固有的蛋白质聚集与年龄的变化

新型杀虫化学对蚊子毒性测试规程

一种研究细胞间分泌信号的近距离培养方法

荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响

Read Article