This content is Free Access.
Capítulos
- 00:05Introduction
- 00:45Image Import and Preprocessing
- 02:45Creating Training Data
- 05:13Using the Segmentation Wizard for Iterative Training
- 06:52Apply the Network
- 07:36Segmentation Manipulation and Cleanup
- 08:16Results: Analysis of the Tomogram of DIV 5 Hippocampal Rat Neuron
- 09:40Conclusion
This is a method for training a multi-slice U-Net for multi-class segmentation of cryo-electron tomograms using a portion of one tomogram as a training input. We describe how to infer this network to other tomograms and how to extract segmentations for further analyses, such as subtomogram averaging and filament tracing.
