Journal
/
Biologia do Desenvolvimento
/
小鼠卵母细胞质微管组织中心的多光子激光消融
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Please note that all translations are automatically generated. Click here for the English version.

小鼠卵母细胞质微管组织中心的多光子激光消融

DOI:
10.3791/64439-v

•

08:24 min

•

November 11, 2022

•

, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Capítulos

  • 00:05Introduction
  • 01:12Mouse Oocyte Collection
  • 02:53Oocyte Microinjection
  • 04:16Oocyte Maturation
  • 05:02Microscope Preparation for Ablation
  • 06:05Ablation of Metaphase Cytoplasmic Microtubule Organizing Centers (mcMTOCs)
  • 06:59Results: Depletion of Metaphase Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
  • 07:35Conclusion

Summary

Tadução automática

Tadução automática

提出了一种优化的方案，该方案能够使用近红外飞秒激光在中期I期间消耗小鼠卵母细胞中的细胞质微管组织中心。

Tags

Multi-photon Laser AblationCytoplasmic Microtubule Organizing CentersMouse OocytesSpindle PositioningMeiosisAneuploidyMTOCsPolar MTOCsMcMTOCsTargeted AblationLive CellPhototoxicityMechanical PerturbationCell Viability

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

基于核仁染色质组织分离鼠标窦卵母细胞与表征

马卵母细胞染色体分离，组蛋白乙酰化和主轴形态分析

手术消融分析在研究涡虫再生眼

多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化

基于神经网络的发育能力或不称职小鼠全生长卵母细胞的识别

斑马鱼内胚细胞中基于共体显微镜的激光消融和再生测定

未成熟费林卵母细胞的最小体积玻璃化

牛早期蚁卵泡卵母细胞的体外培养策略

斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离

从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动

Read Article