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具有长距离纤维排列的微工程3D胶原蛋白水凝胶
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Bioengenharia
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JoVE Journal Bioengenharia
Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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具有长距离纤维排列的微工程3D胶原蛋白水凝胶

DOI:
10.3791/64457-v

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07:12 min

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September 07, 2022

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, , , , , , ,
1Department of Biomedical Engineering, Kate Gleason College of Engineering,Rochester Institute of Technology

Capítulos

  • 00:04Introduction
  • 00:38Fabrication of the Two-Piece Channel and Modular Platform Base
  • 04:28Injecting the COL1 Solution into the Microchannel and Removing the Cover for Cell Culture Applications
  • 06:16Results: Confocal Reflectance Images of COL1 Fibers
  • 06:45Conclusion

Summary

Tadução automática

Tadução automática

该协议演示了使用沿流体流动方向改变几何形状的微流体通道来产生拉伸应变（拉伸）以在3D胶原水凝胶（厚度<250μm）中对齐纤维。由此产生的对齐延伸几毫米，并受拉伸应变率的影响。

Tags

3D Collagen HydrogelsFiber AlignmentMicrofluidicPDMSAminopropyl TriethoxysilaneGlutaraldehydeSurface ModificationTopography

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