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评估小鼠卵母细胞中纺锤体组件检查点完整性
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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评估小鼠卵母细胞中纺锤体组件检查点完整性

DOI:
10.3791/64459-v

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10:09 min

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September 13, 2022

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, ,
1Department of Genetics,Rutgers, The State University of New Jersey, 2Human Genetics Institute of New Jersey, 3Department of Theriogenology, Faculty of Veterinary Medicine,Mansoura University

Capítulos

  • 00:05Introduction
  • 00:51Nocodazole Treatment and Live Imaging
  • 02:41Monitoring the Pattern of Securin-GFP Degradation During Meiotic Maturation
  • 04:42Recruitment of MAD2 at Kinetochores by Immunofluorescence During Meiotic Maturation
  • 07:26Results: Evaluating Spindle Assembly Checkpoint Integrity in Mouse Oocytes
  • 09:33Conclusion

Summary

Tadução automática

Tadução automática

染色体分离错误是卵母细胞的常见特征。因此，研究纺锤体组装检查点为生产健康卵子所需的机制提供了重要线索。本协议描述了三种互补测定，以评估小鼠卵母细胞中的纺锤体组装检查点完整性。

Tags

Keywords: Spindle Assembly CheckpointMeiosisOocyte AneuploidyLive ImagingImmunofluorescenceNocodazoleReversineSecurin-GFPPolar Body ExtrusionAsymmetrical Cytokinesis

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