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Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
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JoVE Journal Medicina
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

DOI:
10.3791/64460-v

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04:21 min

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October 14, 2022

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, , ,
1Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, 2Biochemistry Department, Medicine Faculty,Universidad Nacional Autónoma de Mexico

Capítulos

  • 00:05Introduction
  • 00:37Preparation of the Amniotic Membrane Extract
  • 02:48Results: Determination of Storage Temperature for Amniotic Membrane Extract
  • 03:45Conclusion

Summary

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The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.

Tags

Keywords: LumicanAmniotic MembraneProtein ExtractionBalanced Salt SolutionDispase IIDe-epithelializationLiquid NitrogenProtease InhibitorCentrifugationStorage Temperature

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