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Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
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Biologia
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JoVE Journal Biologia
Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

DOI:
10.3791/64488-v

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06:41 min

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May 19, 2023

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, , , ,
1Hematopoietic Stem Cell Biology and Medical Innovation (HSCBMI), Department of Pediatrics,Kobe University Graduate School of Medicine, 2RIKEN Center for Biosystems Dynamics Research

Capítulos

  • 00:05Introduction
  • 00:52Collection of the Donor Bone Marrow Cells
  • 01:47Supporting Cell Preparation
  • 02:24Gating Setup and Hoxb5pos or Hoxb5neg pHSC Sorting
  • 04:38Results: Functional Analysis of pHSCs Isolated Using Hoxb5 Reporter System
  • 06:10Conclusion

Summary

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We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.

Tags

Hematopoietic Stem CellsHSCLong-term HSCShort-term HSCHoxb5Hoxb5-reporter MiceBone MarrowCell IsolationCell StainingFlow CytometryCell GatingLineage-negativeC-kitSca-1Flk2CD150CD34

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