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Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
JoVE Journal
Biologia
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JoVE Journal Biologia
Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
DOI:

10:35 min

October 06, 2022

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Capítulos

  • 00:04Introduction
  • 00:57Crude Plastoglobule Extraction from Un-Stressed Maize Leaf Tissue
  • 04:41Crude Plastoglobule Extraction from Cyanobacteria
  • 07:06Harvesting Pure Plastoglobules
  • 07:55Cyanobacteria Processing
  • 08:43Results: Isolation of Plastoglobule Lipid Droplets Associated with Various Photosynthetic Organisms
  • 09:36Conclusion

Summary

Tadução automática

A fast and efficient protocol is presented for the isolation of plastoglobule lipid droplets associated with various photosynthetic organisms. The successful preparation of isolated plastoglobules is a crucial first step that precedes detailed molecular investigations such as proteomic and lipidomic analyses.

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