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使用微透析系统实时动态收集意识大鼠的海马细胞外液
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Neurociência
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JoVE Journal Neurociência
Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System
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使用微透析系统实时动态收集意识大鼠的海马细胞外液

DOI:
10.3791/64530-v

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05:43 min

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October 21, 2022

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, , , , ,
1Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Research Institute of Integrated TCM & Western Medicine,Chengdu University of Traditional Chinese Medicine, 3School of Pharmacy,Chengdu University of Traditional Chinese Medicine, 4School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine

Capítulos

  • 00:04Introduction
  • 00:44Brain Microdialysis Probe Implantation Surgery
  • 02:24Microdialysis System Connection and Probe Check
  • 03:15Collection of Hippocampal Extracellular Fluid (HECF) from the Awake Rat
  • 04:02Maintenance of the Microdialysis System and Devices After Sampling
  • 04:36Results: Analysis of HECF Collected Using the Microdialysis Sampling Equipment
  • 04:54Conclusion

Summary

Tadução automática

Tadução automática

这里的协议提供了使用微透析系统从清醒大鼠海马体中细胞外液的详细实时动态采样。

Tags

Hippocampal Extracellular FluidBrain MicrodialysisReal-time Dynamic CollectionConscious RatsCentral Nervous DiseasesStereotaxic Brain LocatorCranial DrillMicrodialysis CasingACSFMulti-channel Swivel ControllerMicrosyringe Pump

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