Journal
/
Pesquisa do Câncer
/
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
JoVE Journal
Pesquisa do Câncer
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Pesquisa do Câncer
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

DOI:
10.3791/64534-v

•

07:46 min

•

October 11, 2022

•

, ,
1Department of Anatomy and Cell Biology, Brody School of Medicine,East Carolina University

Capítulos

  • 00:04Introduction
  • 00:51Isolation of Murine Colonic Crypts
  • 03:19Plating the Isolated Murine Colonic Crypts
  • 04:26Harvesting and Embedding the Colonic Organoids
  • 05:50Results: Growth of Claudin‐7 Deficient Colonoids Throughout the 9 Days of Culture
  • 06:55Conclusion

Summary

Tadução automática

Tadução automática

The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.

Tags

3D CultureColonic CryptsIntestinal Stem CellsEx VivoOrganoid CultureColonMouse ModelClaudin-7Inflammatory Bowel DiseaseColorectal CancerCrypt IsolationEpithelial DissociationCrypt DissociationCell Culture

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/64534?article_type=v

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
check_url/pt/64534?article_type=v

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
check_url/pt/64534?article_type=v

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
check_url/pt/64534?article_type=v

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
check_url/pt/64534?article_type=v

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
check_url/pt/64534?article_type=v

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions
check_url/pt/64534?article_type=v

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
check_url/pt/64534?article_type=v

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
check_url/pt/64534?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
check_url/pt/64534?article_type=v

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Read Article