É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:35Isolation of the Gracilis Muscles
- 01:26Isolation of the Tibialis Anterior (TA) and the Extensor Digitorum Longus (EDL) Muscles
- 03:17Isolation of the Gastrocnemius (GA) and Soleus Muscles
- 04:27Isolation of the Triceps Muscles
- 05:07Isolation of the Masseter Muscles
- 06:08Isolation of the Diaphragm Muscle
- 07:20Muscle Digestion to a Single‐Cell Suspension
- 08:40Results: Purification, Purity Assessment, and Quantitative Measurement of Quiescent Stem Cell Populations from Individual Skeletal Muscles
- 10:47Conclusion
This protocol describes the isolation of muscle stem cells and fibro-adipogenic progenitors from individual skeletal muscles in mice. The protocol involves single muscle dissection, stem cell isolation by fluorescence activated cell sorting, purity assessment by immunofluorescence staining, and quantitative measurement of S-phase entry by 5-ethynyl-2´-deoxyuridine incorporation assay.
