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1Research Institute for Health Sciences and Technologies (SABITA),Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center,Maltepe University, 4Department of Histology and Embryology, School of Medicine,Maltepe University, 5Department of Medical Biology, School of Medicine,Maltepe University, 6School of Medicine,Koc University
Capítulos
- 00:04Introduction
- 01:14Culturing of the Organoids/Spheroids
- 02:27Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
- 04:43Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
- 05:53Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
- 07:25Conclusion
La présente étude décrit des méthodologies pour la culture, la congélation, la décongélation, le traitement, la coloration, l’étiquetage et l’examen de sphéroïdes et d’organoïdes entiers sous divers microscopes, alors qu’ils restent intacts dans un hydrogel dans un dispositif polyvalent.
