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Virusformering og cellebaseret kolorimetrisk kvantificering
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Virus Propagation and Cell-Based Colorimetric Quantification

Virusformering og cellebaseret kolorimetrisk kvantificering

DOI:
10.3791/64578-v

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07:26 min

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April 07, 2023

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, , , ,
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

Capítulos

  • 00:00Introduction
  • 00:43Virus Propagation
  • 02:15Virus Quantification
  • 04:31Staining
  • 05:29Results: Foci Forming Assay for Zika Virus P6-740 in 24 and 96-Well Plates
  • 06:55Conclusion

Summary

Tadução automática

Tadução automática

The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats.

Tags

Virus PropagationCell-based Colorimetric QuantificationZika VirusAntibody RecognitionVirus SerotypesHigh-throughput ApplicationsAutomated Imaging SystemVero CellsCell CultureVirus InoculumVirus QuantificationSerial Dilution

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