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Ekspression af transgener i naturligt blæreurothelium ved hjælp af adenovirusmedieret transduktion
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Biologia
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JoVE Journal Biologia
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Ekspression af transgener i naturligt blæreurothelium ved hjælp af adenovirusmedieret transduktion

DOI:
10.3791/64584-v

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06:01 min

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October 06, 2022

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, , , , ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh School of Medicine

Capítulos

  • 00:04Introduction
  • 00:48Transduction of Rodent Bladder
  • 04:08Results: Transducing the Native Rodent Urothelium for Expression of Transgenes or Downregulation of Endogenous Gene Products
  • 05:33Conclusion

Summary

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Metoder er beskrevet til generering af store mængder rekombinante adenovirus, som derefter kan bruges til at transducere det indfødte gnaverurothelium, hvilket muliggør ekspression af transgener eller nedregulering af endogene genprodukter.

Tags

Keywords: Adenovirus-mediated TransductionBladder UrotheliumCDNASiRNA ExpressionCatheterizationN-dodecyl-beta-D-maltosidePBS WashAnesthetized MouseGene DeliveryUrothelial Gene Expression

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