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Bepaling van de paringsefficiëntie van haploïden in Saccharomyces cerevisiae
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JoVE Journal Biologia
Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
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Bepaling van de paringsefficiëntie van haploïden in Saccharomyces cerevisiae

DOI:
10.3791/64596-v

•

05:39 min

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December 02, 2022

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, ,
1Department of Chemical Engineering,Indian Institute of Technology Bombay, 2Department of Ecology and Evolutionary Biology,University of Michigan, Ann Arbor

Capítulos

  • 00:04Introduction
  • 00:46Patching of Haploids in the Mating Efficiency Grids
  • 01:55Mixing of Haploids and Mating
  • 02:55Plating of Mixed Cells on YPD Agar and Screening for Diploids using Auxotrophic Markers
  • 03:53Results: Quantification and Dynamics of Mating Efficiency in Saccharomyces cerevisiae (Yeast)
  • 05:03Conclusion

Summary

Tadução automática

Tadução automática

In dit werk wordt een robuuste methode beschreven voor de kwantificering van de paringsefficiëntie in de gist Saccharomyces cerevisiae . Deze methode is met name nuttig voor de kwantificering van pre-zygote barrières in soortvormingsstudies.

Tags

Yeast MatingHaploid Mating EfficiencySaccharomyces CerevisiaeMating AssayMating TypeCell GrowthOptical DensityCell SuspensionIncubationMating Grid

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