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Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments
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JoVE Journal Bioengenharia
Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

DOI:
10.3791/64650-v

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11:24 min

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February 03, 2023

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, , , , ,
1Department of Biochemistry and Structural Biology,University of Texas Health Science Center, 2Department of Biomedical Engineering and Chemical Engineering,University of Texas at San Antonio, 3Institute of Regenerative Medicine,University of Texas at San Antonio

Capítulos

  • 00:04Introduction
  • 00:48Isolation of Inguinal, Epididymal, and Posterior Subcutaneous Fat from Rat
  • 03:00Isolation of Microvascular Fragment (MVF)
  • 07:40Casting of the MVF Fibrin Gel
  • 08:37Results: Assessment of the Adipose Tissue Formed from the MVFs
  • 10:46Conclusion

Summary

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Here, we present a detailed protocol outlining the use of microvascular fragments isolated from rodent or human fat tissue as a straightforward approach to engineer functional, vascularized beige adipose tissue.

Tags

3D CultureMicrovascular FragmentsMVFsBeige FatObesityMetabolic DiseaseInguinal FatEpididymal FatPosterior Subcutaneous FatAdipogenesisVascularization

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