Journal
/
Biologia
/
通过荧光共振能量转移研究组蛋白修饰酶与体内转录因子之间的相互作用
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Please note that all translations are automatically generated. Click here for the English version.

通过荧光共振能量转移研究组蛋白修饰酶与体内转录因子之间的相互作用

DOI:
10.3791/64656-v

•

11:33 min

•

October 14, 2022

•

, ,
1Department of Biochemistry and Cell Biology,State University of New York

Capítulos

  • 00:05Introduction
  • 01:02Agroinfiltration
  • 03:21Confocal Microscopy
  • 08:11Results: Specific Interaction of LSH10 with OTLD1 in Nicotiana benthamiana Leaves Detected by SE-FRET and AB-FRET
  • 10:41Conclusion

Summary

Tadução automática

Tadução automática

荧光共振能量转移（FRET）是一种用于检测活细胞中蛋白质相互作用的成像技术。在这里，提出了一种FRET协议来研究组蛋白修饰酶与转录因子的关联，转录因子将它们募集到靶启动子中，用于植物组织中基因表达的表观遗传调控。

Tags

Keywords: FRETProtein-protein InteractionAgroinfiltrationNicotiana BenthamianaHistone Modifying EnzymesTranscription FactorsLiving CellFluorescence Resonance Energy Transfer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/64656?article_type=v

微流体调查可溶性信号介导的相互作用的上皮细胞和细菌的共同文化
check_url/pt/64656?article_type=v

调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em
check_url/pt/64656?article_type=v

短杆菌肽为基础的荧光分析法，确定小分子的修改脂质双层属性的潜在
check_url/pt/64656?article_type=v

成像蛋白质 - 蛋白质相互作用在体内</em
check_url/pt/64656?article_type=v

实时监控的配体 - 受体相互作用的荧光共振能量转移
check_url/pt/64656?article_type=v

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
check_url/pt/64656?article_type=v

快速合成及其与绿色荧光蛋白为基础的记者化学激活的转录因子筛选
check_url/pt/64656?article_type=v

蛋白质 - 蛋白质相互作用的双分子荧光互补的烟草原生质体和叶片可视化
check_url/pt/64656?article_type=v

研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
check_url/pt/64656?article_type=v

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

Read Article