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大鼠胫神经瘤转位模型中的手术和行为测试

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats

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大鼠胫神经瘤转位模型中的手术和行为测试

DOI:

10.3791/64659-v

•

07:23 min

•

January 06, 2023

•

Elisabeth Maria Brakkee, Erick DeVinney, Niels Eijkelkamp, J. Henk Coert

¹Department of Plastic and Reconstructive Surgery,University Medical Center Utrecht, Utrecht University, ²Axogen, ³Center for Translational Immunology,University Medical Center Utrecht, Utrecht University

Capítulos

00:04Introduction
00:40Anesthesia and Preparation
01:12Surgery and Post‐Surgical Treatment
04:30Von Frey Testing of the Plantar Side of the Hind Paws and Neuroma Site
05:25Results: Behavioral Testing of Neuroma Pain and Plantar Hyperalgesia
06:57Conclusion

Summary

Tadução automática

该协议描述了胫神经瘤转位模型，该模型需要胫神经病变，随后近端神经末梢向皮下胫前或外侧位置转位。神经瘤疼痛和足底痛觉过敏的行为测试使用冯弗雷单丝进行定量。

Tags

Neuroma Pain Generalized Pain TNT Model Von Frey Monofilaments Tibial Nerve Tibial Bone Neurovascular Bundle Gastrocnemius Muscles Subcutaneous Tunnel Epineurium Behavioral Testing Rat Surgery

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