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Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
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Neurociência
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JoVE Journal Neurociência
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

DOI:
10.3791/64701-v

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08:25 min

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April 21, 2023

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, , , ,
1Department of Neurology,University of Massachusetts Chan Medical School, 2Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology,University of Massachusetts Chan Medical School

Capítulos

  • 00:04Introduction
  • 00:48Traumatic Brain Injury (TBI) Surgery
  • 01:58Cranial Window Implantation Surgery
  • 06:43Results: Intravital Two‐Photon Imaging
  • 07:41Conclusion

Summary

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Tadução automática

This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.

Tags

Intravital ImagingFluorescent ProteinTraumatic Brain InjuryCranial WindowTwo-photon MicroscopyNeuronal ResponseMechanical StressGene Of InterestVirus PromoterCell TypesOphthalmic OintmentMidline IncisionPeriosteumStereotaxic FrameTBI Impact SiteImpactor TipDental CementLateral MusclesCranial Window Implantation

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