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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

DOI:
10.3791/64718-v

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05:36 min

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February 10, 2023

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,
1Lester & Sue Smith Breast Center,Baylor College of Medicine, 2Department of Medicine,Baylor College of Medicine, 3Department of Molecular & Cellular Biology,Baylor College of Medicine, Houston, TX 77030

Capítulos

  • 00:04Introduction
  • 00:39Syringe and Virus Preparation
  • 01:47Animal Preparation
  • 02:27Intraductal Injection of Virus
  • 03:14Detection of Infected Cells by a Fluorescent Stereomicroscope
  • 03:58Results: Analyzing the Mammary Epithelial Cells After the Intraductal Injection
  • 04:57Conclusion

Summary

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The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

Tags

Keywords: In Vivo Gene DeliveryMammary Epithelial CellsMammary Intraductal InjectionMouse ModelTumor GenesisViral TransductionBromophenol BlueAnesthesiaNipple InjectionMammary Gland Dissection

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