Journal
/
Medicina
/
Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

DOI:
10.3791/64721-v

•

10:33 min

•

November 18, 2022

•

, , , , ,
1School of Pharmacy,Chengdu Medical College

Capítulos

  • 00:04Introduction
  • 00:56Cell Viability Assay
  • 03:02Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
  • 03:37Annexin V-FITC/PI Assay for Apoptosis Rate
  • 05:00Immunofluorescence Detection of Caspase-3 Generation
  • 06:16Immunofluorescence Assay of ROS Level
  • 06:50Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
  • 08:11Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
  • 10:01Conclusion

Summary

Tadução automática

Tadução automática

This protocol provides a combination strategy of two herbs to treat injured PC12 cells. The protocol provides a reference for optimizing the best application mode of traditional Chinese medicine (TCM).

Tags

Keywords: PC12 CellsHypoxiaHerb CombinationIn VitroCell CultureTrypsinFlow CytometryMTT AssayDMSOHomogenous Design Method

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/64721?article_type=v

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
check_url/pt/64721?article_type=v

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation
check_url/pt/64721?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/pt/64721?article_type=v

Dual-phase Cone-beam Computed Tomography to See, Reach, and Treat Hepatocellular Carcinoma during Drug-eluting Beads Transarterial Chemo-embolization
check_url/pt/64721?article_type=v

Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma
check_url/pt/64721?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/pt/64721?article_type=v

Development of Recombinant Proteins to Treat Chronic Pain
check_url/pt/64721?article_type=v

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
check_url/pt/64721?article_type=v

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia
check_url/pt/64721?article_type=v

Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

Read Article