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小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus

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小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化

DOI:

10.3791/64733-v

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06:45 min

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December 16, 2022

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Elien Van Wonterghem*^1,2, Lien Van Hoecke*^1,2, Griet Van Imschoot², Daan Verhaege², Marlies Burgelman², Roosmarijn E. Vandenbroucke²

¹VIB Center for Inflammation Research,VIB, ²Department of Biomedical Molecular Biology,Ghent University

Capítulos

00:05Introduction
00:59Microdissection of the Choroid Plexus Out of the Lateral and Fourth Ventricle
03:47Morphological Analysis of Choroid Plexus Tissue Using Scanning Electron Microscopy (SEM)
04:48Results: Isolation of Choroid Plexus and Analysis of Cellular Structure
05:49Conclusion

Summary

Tadução automática

脉络丛（CP）是神经科学中研究不足的组织，在中枢神经系统的健康和疾病中起着关键作用。该协议描述了用于分离CP的显微切割技术，并使用扫描电子显微镜来获得其细胞结构的整体视图。

Tags

Microdissection Whole Mount Scanning Electron Microscopy Choroid Plexus Brain Ventricles Staining Transcardial Perfusion Mouse Brain Cerebellum Fourth Ventricle

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