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In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

DOI:
10.3791/64749-v

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11:16 min

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February 10, 2023

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1Department of Orthodontics and Dentofacial Orthopedics,Osaka University Graduate School of Dentistry

Capítulos

  • 00:04Introduction
  • 01:04Culture of Mouse Cranial Neural Crest Cells
  • 03:30Preparation of the HA/Col1-Coated Dish
  • 04:49Migration Assay on the HA/Col1-Coated Dish
  • 06:44Results: TMEM2 Expression in NCCs and HA Degradation
  • 09:10Conclusion

Summary

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This protocol outlines an in vitro migration experiment suitable for the functional analysis of the molecules involved in the in vivo migration of neural crest cells into the hyaluronan-rich extracellular matrix.

Tags

Keywords: Hyaluronic AcidExtracellular MatrixNeural Crest Cell MigrationIn Vitro ModelBasement Membrane MatrixO9-1 CellsCell CultureCell MigrationCell AdhesionDrug ScreeningHA Degradation

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