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Thermal Limits Determination for Zooplankton Using a Heat Block
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Biologia
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JoVE Journal Biologia
Thermal Limits Determination for Zooplankton Using a Heat Block

Thermal Limits Determination for Zooplankton Using a Heat Block

DOI:
10.3791/64762-v

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07:16 min

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November 18, 2022

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, ,
1Biology Department,Swarthmore College

Capítulos

  • 00:04Introduction
  • 00:46Fabrication of the Heat Block
  • 01:45Determination of Thermal Gradient Settings
  • 02:47Thermal Exposure and Live-Dead Enumeration
  • 05:30Computation of LT50
  • 06:10Results: Survivorship and Upper Thermal Limits of Larval Sand Dollars
  • 06:48Conclusion

Summary

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The present protocol illustrates the use of commercially available components to generate a stable and linear thermal gradient. Such gradient can then be used to determine the upper thermal limit of planktonic organisms, particularly invertebrate larvae.

Tags

Thermal LimitsZooplanktonHeat BlockGlobal WarmingCritical TemperatureAcclimationOntogenyMarine OrganismsPlanktonTemperature GradientWater BathTemperature ControllerRheostatAluminum BlockThermal PasteTygon TubingThermostat ProbeMicro-centrifuge TubesThermocoupleK-type Electrode

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