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用化学发光法实时检测水稻免疫反应中活性氧的产生
JoVE Journal
Biologia
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JoVE Journal Biologia
Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
DOI:

05:44 min

November 25, 2022

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Capítulos

  • 00:05Introduction
  • 00:36Plant Culture
  • 01:20Tissue Preparation and Pretreatment
  • 02:37Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
  • 03:42Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
  • 05:07Conclusion

Summary

Tadução automática

在这里,我们描述了一种实时检测病原体相关分子模式触发的免疫反应中水稻组织中质外活性氧(ROS)产生的方法。该方法简单、标准化,可在受控条件下产生高度可重复的结果。

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