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大麻中腺头柄和无柄毛状体的非水分离和富集
JoVE Journal
Genética
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JoVE Journal Genética
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
DOI:

08:18 min

May 12, 2023

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Capítulos

  • 00:04Introduction
  • 00:40Setup for the Initial Separation of Trichomes from the Plant Material
  • 02:08Separation of Trichomes from the Plant Material
  • 03:24Separation of Glandular Capitate Stalked and Sessile Trichomes from Other Trichome Types
  • 05:13Passing Trichomes Through Micro-Sieves of Decreasing Pore Size and Collection of the Desired Trichomes
  • 06:14Results: High-Throughput Isolation & Enrichment of Glandular Capitate Stalked & Sessile Trichomes from Cannabis sativa
  • 07:29Conclusion

Summary

Tadução automática

提出了一种方案,用于方便和高通量地从 大麻中分离和富集腺头柄和无柄毛状体。该方案基于仅使用液氮、干冰和尼龙筛对毛状体进行干燥、非缓冲提取,适用于 RNA 提取和转录组学分析。

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