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カンナビスサティバからの腺頭状茎および固着毛状突起の非水性分離と濃縮
JoVE Journal
Genética
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JoVE Journal Genética
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
DOI:

08:18 min

May 12, 2023

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Capítulos

  • 00:04Introduction
  • 00:40Setup for the Initial Separation of Trichomes from the Plant Material
  • 02:08Separation of Trichomes from the Plant Material
  • 03:24Separation of Glandular Capitate Stalked and Sessile Trichomes from Other Trichome Types
  • 05:13Passing Trichomes Through Micro-Sieves of Decreasing Pore Size and Collection of the Desired Trichomes
  • 06:14Results: High-Throughput Isolation & Enrichment of Glandular Capitate Stalked & Sessile Trichomes from Cannabis sativa
  • 07:29Conclusion

Summary

Tadução automática

カンナビスサティバからの腺頭状茎および固着毛状突起の便利で高スループットの分離と濃縮のためのプロトコルが提示されています。このプロトコルは、液体窒素、ドライアイス、ナイロンふるいのみを使用したトリコームの乾燥した非緩衝抽出に基づいており、RNA抽出およびトランスクリプトーム解析に適しています。

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