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小鼠自身免疫性糖尿病过继转移模型中移植物存活的生物发光监测
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Biologia
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JoVE Journal Biologia
Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
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小鼠自身免疫性糖尿病过继转移模型中移植物存活的生物发光监测

DOI:
10.3791/64836-v

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10:03 min

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November 18, 2022

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, , ,
1Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center,Harvard Medical School, 2Section for Immunology, Joslin Diabetes Center,Harvard Medical School

Capítulos

  • 00:04Introduction
  • 00:47Transplantation of NIT-1 Cells into Non-Obese Diabetic (NOD)- Severe Combined Immunodeficient (scid) Mice
  • 03:15Isolation and Purification of Autoreactive Splenocytes
  • 05:33Intravenous Injection of Diabetic Splenocytes via the Lateral Tail Vein
  • 07:17In Vivo Bioluminescent Imaging of NIT-1 Grafts
  • 08:28Results: Monitoring Graft Survival in NOD-scid Mice by Bioluminescent Imaging
  • 09:37Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了一种直接和微创的方法，用于在非肥胖糖尿病（NOD）严重联合免疫缺陷小鼠中移植和成像NIT-1细胞，这些小鼠受到从自发糖尿病NOD小鼠纯化的脾细胞的挑战。

Tags

Bioluminescent MonitoringGraft SurvivalAdoptive TransferAutoimmune DiabetesMiceCell CultureTransplantImagingTail Vein InjectionIsofluraneSubcutaneous InjectionCell Suspension

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