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Differentiering af humane pluripotente stamceller i insulinproducerende øklynger
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differentiering af humane pluripotente stamceller i insulinproducerende øklynger

DOI:
10.3791/64840-v

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08:41 min

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June 23, 2023

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, , , , , ,
1Department of Cellular & Physiological Sciences, Life Science Institute,University of British Columbia, 2School of Biomedical Engineering,University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery,University of British Columbia

Capítulos

  • 00:00Introduction
  • 00:35Differentiation of hPSCs into Pancreatic Progenitor Cells
  • 02:48Aggregation into Pancreatic Progenitor Clusters Using 400 μm Diameter Microwell Plates
  • 04:20Differentiation of Kit-Derived Pancreatic Progenitor Clusters into hPSC-Islets
  • 06:07Results: Generation of hPSC-Islets Capable of Secreting Insulin upon Glucose Challenge
  • 08:07Conclusion

Summary

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Differentieringen af stamceller i øceller giver en alternativ løsning til konventionel diabetesbehandling og sygdomsmodellering. Vi beskriver en detaljeret stamcellekulturprotokol, der kombinerer et kommercielt differentieringssæt med en tidligere valideret metode til at hjælpe med at producere insulinudskillende, stamcelleafledte øer i en skål.

Tags

Keywords: Human Pluripotent Stem CellsInsulin-producing Islet ClustersStatic Suspension CulturePancreatic ProgenitorsIslet-like ClustersHESC-qualified MatrigelCell DissociationStem Cell CultureStage 1A MediumStage 1B MediumStage 2A MediumStage 2B MediumStage 3 MediumStage 4 MediumAnti-adherence Rinsing SolutionMicrowell Plate

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