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The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation
JoVE Journal
Biologia
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JoVE Journal Biologia
The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation
DOI:

05:38 min

June 23, 2023

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Capítulos

  • 00:00Introduction
  • 00:57Preparing the Eggs for Incubation and Opening the Eggshell on Day 3 of Chick Embryo Development (ED)
  • 02:38Grafting Frozen-Thawed Human Ovarian Tissue to the CAM and Harvesting the Grafts
  • 03:33Results: Development of a CAM Xenografting Model for Human Ovarian Tissue
  • 04:37Conclusion

Summary

Tadução automática

Here, we describe a protocol for developing a chick chorioallantoic membrane (CAM) xenografting model for human ovarian tissue and demonstrate the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period.

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