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胰腺组织解剖以分离活的单细胞
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Pancreatic Tissue Dissection to Isolate Viable Single Cells
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胰腺组织解剖以分离活的单细胞

DOI:
10.3791/64871-v

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08:04 min

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May 26, 2023

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, ,
1The Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada, Faculty of Medicine,The Hebrew University of Jerusalem

Capítulos

  • 00:05Introduction
  • 01:07Pancreatic Dissection
  • 02:09Enzymatic and Mechanical Dissociation of the Pancreas
  • 05:49Results: FACS Analysis of Isolated Single-Cell Suspension and scRNA-Sequencing
  • 07:12Conclusion

Summary

Tadução automática

Tadução automática

胰腺化生细胞是引起胰腺肿瘤的恶性细胞的前体。然而，分离完整的活胰腺细胞具有挑战性。在这里，我们提出了一种有效的胰腺组织解离方法。然后，这些细胞可用于单细胞RNA测序（scRNA-seq）或二维或三维共培养。

Tags

Viable Single CellsAcinar CellsPancreatic TumorsBiomarkersTreatmentsEuthanized MouseAbdominal CavityPancreasSpleenDissociation BuffersFetal Bovine SerumHank’s Balanced Salt SolutionNoyes ScissorsScalpelCentrifuge

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