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1Department of Medical, Oral and Biotechnological Sciences,“G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST),“G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences,University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti,Università degli Studi di Milano, 6Department of Pharmaceutical Sciences,Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases,Centro Cardiologico Monzino IRCCS
Capítulos
- 00:04Introduction
- 00:45Sample Preparation and Enzymatic Digestion
- 01:47Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
- 02:32Sedimentation of Cell Clusters and Cell Seeding
- 03:16Results: Purification and Characterization of Freshly Isolated HLMVECs
- 04:30Conclusion
Obwohl die Isolierung von pulmonalen Endothelzellen eine Herausforderung darstellt, ist sie für Studien zur Lungenentzündung unerlässlich. Das vorliegende Protokoll beschreibt ein Verfahren zur hochwirksamen, hochreinen Isolierung von makrovaskulären und mikrovaskulären Endothelzellen.
