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In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology
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JoVE Journal Química
In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology

In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology

DOI:
10.3791/64902-v

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13:17 min

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September 29, 2023

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, , , , , , , , , , , ,
1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics

Capítulos

  • 00:00Introduction
  • 01:22IC50 Evaluation for LMB002 (“ring-closed” and “ring-open” forms) Using 2D and 3D LLC Cell Cultures
  • 03:062D Cell Culture Experiment—Adding the Compounds (Day 2)
  • 04:142D Cell Culture Experiment—Staining and Imaging (Days 2–5)
  • 04:453D Cell Culture Experiment
  • 05:46Determination of Photoswitching Efficiency in a Tissue Surrogate
  • 07:46In Vivo Anticancer Efficacy Determination
  • 09:39Representative Results
  • 12:40Conclusion

Summary

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This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo efficacy.

Tags

Photocontrolled CompoundsPhotoswitchable DrugsPhotopharmacologyIn VitroIn VivoCancerCell Viability AssayLewis Lung CarcinomaDMSODMEMPhotoirradiationLaser

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